最為理想的檢測武漢肺炎方法,當然是要準確同快速兩者兼備,不需任何儀器家用便能做到為之更佳。494Please respect copyright.PENANA0Wby8h27Tm
494Please respect copyright.PENANA2M5F9IAifO
494Please respect copyright.PENANA93uXPFMct8
現今最為標準的檢測方法RT-QPCR,但整個過程接近耗時2小時,而且亦需要實驗室PCR儀器及專業人士確認結果的可信性。494Please respect copyright.PENANA8BAvDv4gxa
494Please respect copyright.PENANAWtNZEJscZJ
較為快捷但並未全面通行的方法為RT-LAMP,整個過程約耗時30-50分鐘,可套用不同方法展示結果,最高靈敏度比RT-QPCR約低10倍。494Please respect copyright.PENANAdWn6lkFPck
494Please respect copyright.PENANAyKX1jgL8HD
整個過程受著需要在攝氏65度運行的限制,距離家用化仍擁有著些許的不便。494Please respect copyright.PENANA15AVB4MeU2
494Please respect copyright.PENANAdVnR6pm69E
494Please respect copyright.PENANAnpiLAJQOvc
(圖2)最近有科學家研發一套名為SENSR的檢測方法(Ref1),耗時約30-50分鐘,但全程只需在攝氏37度運行!494Please respect copyright.PENANAJnnU2TfnyH
494Please respect copyright.PENANAeTVzUdhGSN
494Please respect copyright.PENANAIAzOh92DEg
他們嘗試利用最為準確的RT-QPCR方法作出對比,40個樣本之中,有39個達一致結果。494Please respect copyright.PENANA1Zaup4J5TT
整個檢測方法主要包含四大組件,如圖中所示。494Please respect copyright.PENANAhskdW3URIQ
494Please respect copyright.PENANAjpH4Zgi2PA
494Please respect copyright.PENANADnSLniaThE
(圖3)經過精心的設計,兩隻Probes會辨認武肺病毒RNA前後二段位置。494Please respect copyright.PENANAkRBOGffeq7
兩隻Probes同時辨認成功後,名為SplintR ligase的蛋白質便會將兩隻Probes連成一起。494Please respect copyright.PENANAQqofPjjPC9
494Please respect copyright.PENANACAtfVu8QHf
494Please respect copyright.PENANAnL2hWVhUhu
494Please respect copyright.PENANA7kcqGoqFxu
(圖4)連成一起的Probe將會被T7 RNA polymerase 利用,生產出特殊結構的物質。494Please respect copyright.PENANA5NnmtNjAP5
494Please respect copyright.PENANA9cFjmPqcvK
494Please respect copyright.PENANAvahqiEcQo0
這些特殊結構物質會Fluroescence Dye結合,發出螢光,而沒有與特殊結構物質結合的Fluroescence Dye則不會發出螢光。494Please respect copyright.PENANAvEE6jCspez
494Please respect copyright.PENANAQMMPstbb5u
494Please respect copyright.PENANAmVL8sHnHFg
(圖5)只要將病人口水及能夠打破病毒外層的化學物與檢測方法組件放在一起,在攝氏37度的環境下,半小時後便能憑著螢光強度獲取結果。494Please respect copyright.PENANA5jpHnKp9xp
494Please respect copyright.PENANA2CHNG4Nw9v
494Please respect copyright.PENANA7e27mr6VpL
沒有螢光,意指沒有武肺病毒的存在。494Please respect copyright.PENANARTce8IRH2A
而螢光越強,則代表武肺病毒數量就越多。494Please respect copyright.PENANAXFVFD8YvSI
494Please respect copyright.PENANAgGJb8eGGDp
若然整套檢測方法能長久地與RT-QPCR的結果吻合的話,甚至能改良至更短時間完成的話,那些健康碼或健康護照的系統便完全成為被科技淘汰、多餘無用的產物。494Please respect copyright.PENANAIOAlTC0iOh
494Please respect copyright.PENANAWgnglgIbmP
494Please respect copyright.PENANAfP9a0tGSGs
如果想我快啲出文的話,請入去Penana網站科普專欄–薯不揀單俾一個like以及Bookmark,這對我非常之重要。494Please respect copyright.PENANAzkfB77rL2Y
494Please respect copyright.PENANAx8L34SGS6W
Link: https://www.penana.com/story/66244/%E7%A7%91%E6%99%AE%E5%B0%88%E6%AC%84-%E8%96%AF%E4%B8%8D%E6%8F%80%E5%96%AE/toc494Please respect copyright.PENANAqhWSCfMEy9
494Please respect copyright.PENANA3CFkWanyeG
同時亦可以在Penana網站裡按「買一嚿/半嚿薯仔」,以課金的方式推動我繼續搜羅資料寫文,多謝。494Please respect copyright.PENANAhF7GIZ4Ylv
494Please respect copyright.PENANAXjbaLwDm0Q
Link:494Please respect copyright.PENANAzp5Fo5Wb39
https://www.penana.com/user/27349/%E8%96%AF%E4%BB%94%E8%81%9E%E8%8C%B1%E8%8E%89/portfolio494Please respect copyright.PENANA4XsNV6dyQd
494Please respect copyright.PENANA4x2hiErVmS
494Please respect copyright.PENANANaDClkITFb
有興趣接收科學資訊的話,歡迎follow IG: potatopigtongue。494Please respect copyright.PENANA1X5PQrhQh5
494Please respect copyright.PENANAbSmBPLCfau
494Please respect copyright.PENANAqUMnZxAAxT
Ref1:https://www.nature.com/articles/s41551-020-00617-5.epdf?sharing_token=jyB1FrYxTMs2zz7531NEC9RgN0jAjWel9jnR3ZoTv0Oek8rnwPluVmxh8oas6RXA_yYuSqspJD8rPlYkIjcQAnYsXIpSHTS1rRw2pBOABq2RcPNywJhYXk5RtoOUasrjInaGxrHnZw-U2KJCVSypN2TLCfr8XdJlRq3q0or2Vs8%3D